Recombinant Human UCHL5, His-tagged

• Cat.No.: UCHL5-1519H
• Product Overview: Recombinant human UCHL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-329aa).

Specification

• Cat. No.: UCHL5-1519H
• Description: Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves "Lys-48"-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
• Molecular Weight: 39.7kDa (349aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Biological activity: Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.
• Activity Assay: 1. Prepare a 100ul of recombinant UCH-L5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC - 96 Well Polystyrene Microplate, black - Fluorescent plate reader
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UCHL5 ubiquitin carboxyl-terminal hydrolase L5 [ Homo sapiens ]
• Official Symbol: UCHL5
• Synonyms: UCHL5; UCH37; CGI-70; INO80R; AD-019; UCH-L5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; OTTHUMP00000033565; OTTHUMP00000033566; OTTHUMP00000033567; OTTHUMP00000033568; OTTHUMP00000033570; OTTHUMP00000033571; OTTHUMP00000033754; INO80 complex subunit R; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; EC 3.4.19.12
• Gene ID: 51377
• mRNA Refseq: NM_015984
• Protein Refseq: NP_057068
• MIM: 610667
• UniProt ID: Q9Y5K5
• Chromosome Location: 1q32
• Function: cysteine-type peptidase activity; endopeptidase inhibitor activity; omega peptidase activity; peptidase activity; proteasome binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity