| Cat. No. : |
Vcam1-4013M |
| Description : |
Mouse vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two isoforms of mouse VCAM1 have been identified, referred to as single-pass type I transmembrane (short) isoform 1 containing seven C2-type I g-like domains and GPI-anchored isoform 2. Mouse VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. |
| Source : |
Mouse |
| Host : |
Human Cells |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements. |
| Purity : |
> 97 % as determined by SDS-PAGE |
| Bio-activity : |
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells / well are added to mouse VCAM1 coated plates ( 10 μg/mL with 100 μL/well ) , approximately 70% - 80% cells will adhere after 1 hour incubation at RT |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70μ |
| Predicted N terminal : |
Phe 25 |
| Molecular Mass : |
The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation. |
| Storage : |
Store it under sterile conditions at -70μ. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
