Native Human Adiponectin

• Cat.No.: ADIPOQ-215H
• Product Overview: 226AA. MW: 24.54 kDa (calculated without glycosylation).

Specification

• Description: ADIPOQ (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene.
• Source: Human pooled serum
• Species: Human
• Molecular Mass: 24.54 kDa (calculated without glycosylation).
• Protein length: 226AA
• AA Sequence: ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN
• Applications: Cell culture and/or animal studies; ELISA; Immunological methods; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; Gel permeation chromatography to determine purity and oligomeric state of the native protein; LAL to determine quantity of endotoxin; Immunoreactivity is confirmed using monoclonal antibodies specific to human adiponectin
• Storage: Store lyophilized protein at -20°C. Lyophilized pro-tein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Re-constituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Gene Information

• Gene Name: ADIPOQ adiponectin, C1Q and collagen domain containing [ Homo sapiens ]
• Official Symbol: ADIPOQ
• Synonyms: ADIPOQ; adiponectin, C1Q and collagen domain containing; ACDC, adipocyte, C1Q and collagen domain containing; adiponectin; ACRP30; AdipoQ; adipose most abundant gene transcript 1; apM1; GBP28; gelatin-binding protein 28; adipose specific collagen-like fa
• Gene ID: 9370
• mRNA Refseq: NM_001177800
• Protein Refseq: NP_001171271
• MIM: 605441
• UniProt ID: Q15848
• Chromosome Location: 3q27
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Developmental Biology, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem
• Function: cytokine activity; eukaryotic cell surface binding; hormone activity; identical protein binding; protein binding; protein homodimerization activity; receptor binding; sialic acid binding