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Recombinant Human COL2A1 protein(Asp1242-Leu1487), His-tagged

Cat.No. : COL2A1-3929H
Product Overview : Recombinant Human COL2A1 isoform 1 (P02458-2)(Asp 1242-Leu 1487), known as chondrocalcin, was expressed in HEK293, with a polyhistidine tag at the N-terminus.
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Source : HEK293
Species : Human
Tag : N-His
Protein length : Asp1242-Leu1487
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human COL2A1 (aa 1242-1487) , known as chondrocalcin consists of 265 amino acids and has a calculated molecular mass of 29.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 37 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 92 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Publication :
Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis (2021)
Gene Name : COL2A1 collagen, type II, alpha 1 [ Homo sapiens ]
Official Symbol : COL2A1
Synonyms : COL2A1; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital) , SEDC; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; AOM; ANFH; SEDC; COL11A3; MGC131516;
Gene ID : 1280
mRNA Refseq : NM_001844
Protein Refseq : NP_001835
UniProt ID : P02458

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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