Recombinant Human Dynein Light Chain 2, LC8-type2, His-tagged

• Cat.No.: DYNLL2-3547H
• Product Overview: Recombinant Human DYNLL2(1-89aa) produced in E.coli contains His tag at N-terminus and is purified by using conventional chromatography techniques.

Specification

• Cat. No.: DYNLL2-3547H
• Description: Recombinant Human DYNLL2(1-89aa), His-tagged is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. DYNLL2 is a highly conserved eukaryotic hub protein with dozens of binding partners and various functions beyond being a subunit of dynein and myosin Va motor proteins. Recombinant human DYNLL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and have a total molecular mass of 12.5 kDa (109aa), confirmed by MALDI-TOF.
• Source: E. coli.
• Purity: > 90% by SDS – PAGE.
• Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.2M NaCl.
• Molecular Weight: 12.5 kDa (109aa), confirmed by MALDI-TOF.
• Concentration: 1mg/ml (determined by Bradford assay).
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSDRKAVIKN ADMSEDMQQD AVDCATQAME KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFK- SG
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: DYNLL2 dynein, light chain, LC8-type 2 [ Homo sapiens ]
• Synonyms: dynein, light chain, LC8-type 2; DLC8b; RSPH22; DLC2; MGC17810; radial spoke 22 homolog; Dlc2;dynein light chain 2, cytoplasmic; DNCL1B;Dynein light chain LC8-type 2; 8 kDa dynein light chain b; DYNLL2
• Gene ID: 140735
• mRNA Refseq: NM_080677
• Protein Refseq: NP_542408
• MIM: 608942
• UniProt ID: Q96FJ2
• Chromosome Location: 17q22
• Pathway: Activation of BH3-only proteins; Activation of BMF and translocation to mitochondria; Apoptosis; Intrinsic Pathway for Apoptosis; Vasopressin-regulated water reabsorption
• Function: cytoskeletal protein binding; motor activity; protein binding