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Cat. No.
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CPE06
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Description
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Deoxyribonuclease I (DNase I) is an endonuclease that cuts preferentially next to pyrimidine nucleotides, yielding 5"-phosphate-terminated oligodeoxynucleotides ~ 4 bases in length. It is active on both single and double-stranded DNA and may produce double-stranded breaks in the presence of Mn2+.
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CAS No.
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9003-98-9
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Synonym
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pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; DNase I; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease
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Molecular Weight
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40,000 Da
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Composition
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There are four deoxyribonucleases of Bovine Pancreas: A, B, C, and D; DNase A is the predominant form.
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Optimum pH
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7.8.
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Activators
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DNase I is activated by bivalent metals. Maximum activation is attained with Mg2+ plus Ca2+. It has been indicated that a metallosubstrate, such as Mg salt of DNA might be necessary.
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Inhibitors
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Citrate completely inhibits magnesium-activated but not manganese-activated enzyme. DNase I is inhibited by chelating agents such EDTA, and sodium dodecyl sulfate.
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Stabilizers
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The most likely proteolytic contaminant of DNase I is chymotrypsin B. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+. Diisopropylfluorophosphate (DFP) may also be used to inhibit contaminating proteases.
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Product Specification(Ⅰ)
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Appearance
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White or pale yellow lyophilized powder
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Activity(K.P.)
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≥ 2,000 Kunitz units/mg material
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Loss on drying(K.P.)
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≤ 0.3%
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Residue on ignition(K.P.)
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≤ 1.0%
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Unit definition: One unit is the amount of enzyme that produces an absorbance increase of 0.001 per minute at 260 nm under standard assay conditions at 25°C with DNA as a substrate.
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Product complies with K.P.
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Specification(Ⅱ)
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Activity (Sigma-aldrich)
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≥ 500 Kunitz units/mg material
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Specification(Ⅲ)
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Activity (Sigma-aldrich)
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≥ 2,000 Kunitz units/mg material
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Stability/Storage
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The lyophilized enzyme is stable for 2-5 years when stored at 5°C.
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Download Datasheet:
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