Catalog # or name ...
From Bovine Pancreas
Deoxyribonuclease I (DNase I) is an endonuclease that cuts preferentially next to pyrimidine nucleotides, yielding 5"-phosphate-terminated oligodeoxynucleotides ~ 4 bases in length. It is active on both single and double-stranded DNA and may produce double-stranded breaks in the presence of Mn2+.
pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; DNase I; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease
There are four deoxyribonucleases of Bovine Pancreas: A, B, C, and D; DNase A is the predominant form.
DNase I is activated by bivalent metals. Maximum activation is attained with Mg2+ plus Ca2+. It has been indicated that a metallosubstrate, such as Mg salt of DNA might be necessary.
Citrate completely inhibits magnesium-activated but not manganese-activated enzyme. DNase I is inhibited by chelating agents such EDTA, and sodium dodecyl sulfate.
The most likely proteolytic contaminant of DNase I is chymotrypsin B. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+. Diisopropylfluorophosphate (DFP) may also be used to inhibit contaminating proteases.
White or pale yellow lyophilized powder
≥ 2,000 Kunitz units/mg material
Loss on drying(K.P.)
Residue on ignition(K.P.)
Unit definition: One unit is the amount of enzyme that produces an absorbance increase of 0.001 per minute at 260 nm under standard assay conditions at 25°C with DNA as a substrate.
Product complies with K.P.
≥ 500 Kunitz units/mg material
The lyophilized enzyme is stable for 2-5 years when stored at 5°C.
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