Catalog # or name ...
From Calf Spleen
Adenosine deaminase (ADA) is a purine catabolic enzyme ubiquitous in mammalian tissue. In vitro it catalyzes deamination of both adenosine and 2"-deoxyadenosine to inosine and 2"-deoxyinosine respectively.
Adenosine + H2O → Inosine + NH3.
Adenosine aminohydrolase; deoxyadenosine deaminase.
Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0;
One unit will deaminate one micromole of adenosine to inosine per minute at pH 7.4 at 25℃.
32.5~33 kDa (SDS-PAGE)
The enzyme is a glycoprotein containing galactosamine and glucosamine. Its composition shows a predominance of acidic amino acids.
<60℃ (pH7.4, 30min)
Ag+, Hg2+, Cu2+, sulfhydryl reagents (pCMB and methyl mercuric nitrate) are strongly inhibiting. The even stronger inhibiting effect of TPCK and DFP suggests that histidine or serine or both might play a role in the active site of the enzyme.
The enzyme preparation may be diluted in water or buffer to a protein concentration of 1mg/ml, producing a clear, colorless solution.
Purine metabolism; Nucleotide metabolism
adenosine deaminase activity; hydrolase activity; protein binding; zinc ion binding
Ribbon diagram of bovine adenosine deaminase.
Zinc ion visible at center. From PDB 1VFL
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