Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human VAMP3 Monoclonal Antibody

Mouse Anti-Human VAMP3 Monoclonal Antibody

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GST(1)
His(5)
N/A(1)
None(1)
Cat. No.:
CAB-442 MH
ProductOverview:
Monoclonal Antibody to Human vesicle-associated membrane protein 3 (cellubrevin)(VAMP3)
Antigendescription:
Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.
Immunogen:
Recombinant human cellubrevin (1-77aa) purified from E. coli.
Clone Number:
MAFB4160
Isotype:
Mouse IgG2b heavy chain and k light chain.
Clone:
Anti-human VAMP3 mAb, clone MAFB4160, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant hu-man cellubrevin protein.
Concentration:
1mg/ml.
Form:
Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
Applications:
ELISA, WB.
Usage:
The antibody has been tested by ELISA and West-ern blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recom-mended dilution range for Western blot analysis is 1:1000 ~ 1:2000. Recommended starting dilution is 1:1000.
Storage:
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Western Blot Analysis:
The extracts of mouse lung tissue(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cellubrevin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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