Cat. No. : |
CAB-442 MH |
ProductOverview : |
Monoclonal Antibody to Human vesicle-associated membrane protein 3 (cellubrevin)(VAMP3) |
Antigendescription : |
Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin. |
Immunogen : |
Recombinant human cellubrevin (1-77aa) purified from E. coli. |
Clone Number : |
MAFB4160 |
Isotype : |
Mouse IgG2b heavy chain and k light chain. |
Clone : |
Anti-human VAMP3 mAb, clone MAFB4160, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant hu-man cellubrevin protein. |
Concentration : |
1mg/ml. |
Form : |
Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
Applications : |
ELISA, WB. |
Usage : |
The antibody has been tested by ELISA and West-ern blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recom-mended dilution range for Western blot analysis is 1:1000 ~ 1:2000. Recommended starting dilution is 1:1000. |
Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Western Blot Analysis : |
The extracts of mouse lung tissue(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cellubrevin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. |