Catalase

Cat. No.

CED004

Description

Catalase is a common enzyme found in nearly all living organisms, where it functions to catalyze the decomposition of hydrogen peroxide to water and oxygen. Catalase has one of the highest turnover numbers of all enzymes; one molecule of catalase can convert millions of molecules of hydrogen peroxide to water and oxygen per second. Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four porphyrin heme (iron) groups that allow the enzyme to react with the hydrogen peroxide. The optimum pH for catalase is approximately 7, while the optimum temperature varies by species.

Appearance

green liquid

CAS Number

9001-05-2

Specific Activity

≥ 10KU/mg protein

Reaction

2 H₂O₂ = O₂ + 2 H₂O

Synonyms

hydrogen-peroxide:hydrogen-peroxide oxidoreductase; equilase; caperase; optidase; catalase-peroxidase; CAT.

Unit definition

One unit decomposes one micromole of H₂O₂ per min at pH 7.0 at 25℃.

Molecular Weight

247kDa (Gel filtration)

Isoelectric point

4.5

Michaelis constant

4.5×10-3M

Optimum temperature

35℃

Optimum pH    

8.0

pH Stability

5.0~9.0 (25℃, 20hr)

Thermal Stability

< 40℃ (pH7.0, 20min)

Inhibitors

Zn2+,Ag+,Hg2+

Preparation Instructions

The enzyme is diluted with 50mM potassium phosphate buffer, pH 7.0 for activity assay.

Storage Temperature

-20℃