Monoclonal Antibody to MAT2A.
MAT2A is an important enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver,MAT2A expression associates with growth,dedifferentiation,and cancer. NF-kappa B and AP-1 are necessary for basal MAT2A expression in HepG2 cells and mediate the increase in MAT2A expression in response to TNF-alpha. Up-regulation of MAT2A provides growth improvement and s-adenosylmethionine and methylthioadenosine thus can block mitogenic signaling in colon cancer cells. Lower expression of both MAT2A and MAT2beta and interfere with leptin signaling in liver cancer cells.
Anti-human MAT2A mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human MAT2A amino acids 1-395 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
MAT2A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
1mg/ml containing PBS,pH-7.4,& 0.1% Sodium Azide.
Stable for 1 year at -20 °C and 3 months at 4 °C. For maximum recovery of the product,centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.