Catalog # or name ...
Diazyme Glucose-6-phosphate Dehydrogenase is extensively purified from microorganisms. The enzyme utilizes both NAD+ and NADP+ as co-factors. The enzyme is useful for the enzymatic assay of NAD+ or NADP+ and Glucose-6-phosphate, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase.
D-Glucose-6-phosphate + NAD(P) → D-Glucono -lactone-6-phosphate + NAD(P)H2.
glucose-6-phosphate dehydrogenase; NADP-glucose-6-phosphate dehydrogenase; Zwischenferment; D-glucose 6-phosphate dehydrogenase; glucose 6-phosphate dehydrogenase (NADP); NADP-dependent glucose 6-phosphate dehydrogenase; 6-phosphoglucose dehydrogenase; Entner-Doudoroff enzyme; glucose-6-phosphate 1-dehydrogenase;G6PDH; GPD; D-glucose-6-phosphate: NADP+1-oxidoreductase; OTTHUMP00000026034 ; G6PD1; G6PD_HUMAN; G6PD;EC 184.108.40.206;glucose-6-phosphate dehydrogenase.
White amorphous powder, lyophilized.
150U/mg-solid or more(NAD+)
One unit caused the formation of one micromole of NADH per minute at pH7.8 at 30°C.
104,000 dalton (two subunits of approx. 55,000)
NAD+ linked :1.06×10-4 M (NAD+); 5.27×10-5 M (G-6-P)
NADP+linked :5.69×10-6M (NADP+); 8.1×10-5M (G-6-P)
pH 5.5~7.5 (30℃, 17hr)
below 37℃(pH 8.0, 30min)
Acyl-CoA, ATP, mental ions etc
Dissolve the enzyme preparation in ice-cold enzyme diluent (5mM Tris-HCl buffer, pH7.5, containing 0.1% of bovine serum albumin) and dilute to 0.05-0.20U/ml with the same buffer, immediately before activity assay
Stable at -20℃ for at least 6 months(powder form)
Stable at 5℃ for at least 6 months(liquid form)
G6PD (Homo sapiens)
Glutathione metabolism; Pentose phosphate pathway; Metabolism of carbohydrates.
glucose-6-phosphate dehydrogenase activity; oxidoreductase activity; binding.
Available structures: 1qki, 2bh9, 2bhl
45-16 Ramsey Road, Shirley, NY 11967, USA | TEL:1-631-559-9269 FAX:1-631-938-8127 | EMAIL:email@example.com
Copyright 2013 (c) Creative Biomart. All rights reserved. et: 31.24 ms