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Cat. No.
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CED013
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Description
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The activity of glycerol kinase is found widely in nature. In microorganisms GK makes possible the utilization of glycerol as a carbon source. In mammals the enzyme represents a juncture of sugar and fat metabolism; The enzyme is important to the clinical chemist in the determination of glycerol. GK is also useful in the assay of glyceraldehydes and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride.
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CAS No.
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9030-66-4
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Synonym
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glycerokinase; GK; ATP:glycerol-3-phosphotransferase; glycerol kinase (phosphorylating); glyceric kinase; GK1; GKD; GK_HUMAN; glycerol kinase; glycerol kinase deficiency.
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Reaction
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ATP + glycerol = ADP + sn-glycerol 3-phosphate
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Physical form
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White powder, lyophilized
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Specific Activity
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≥ 250U/mg protein
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Unit definition
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One unit will convert one micromole of glycerol to glycerol-3-phosphate per min at pH 7.9 at 37℃.
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Molecular Weight
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55 kDa (SDS-PAGE)
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Composition
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the enzyme is composed of four identical subunits.
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Isoelectric point
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5.1
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Michaelis constant
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3.8×10-5M (Glycerol)
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Optimum pH
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8.0
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Optimum temperature
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80℃
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pH Stability
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5.0~10.0 (30℃, 20hr)
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Storage Temperature
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-20℃
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Thermal Stability
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< 60℃ (pH7.5, 30min)
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Inhibitors
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Ag+,Hg2+
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Preparation Instructions
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The enzyme is reconstituted in 20mM potassium phosphate buffer, pH 7.5 for activity assay.
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Gene Information
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Gene Name
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GK (human)
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GeneID
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2710
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mRNA Refseq
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NM 001128127
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Protein Refseq
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NP_001121599
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MIM
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300474
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UniProt ID
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P32189
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Chromosome Location
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Xp21.3
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Pathway
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Glycerolipid metabolism; PPAR signaling pathway; Lipid and lipoprotein metabolism.
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Function
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ATP binding; glycerol kinase activity; nucleotide binding; transferase activity.
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