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Cat. No.
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CED020
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Description
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Purine nucleoside phosphorylase (also known as PNPase) is an enzyme (EC 2.4.2.1) involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine, in each case creating ribose phosphate. NP encodes the enzyme purine nucleoside phosphorylase that together with adenosine deaminase (ADA) serves a key role in purine catabolism, referred to as the salvage pathway. Mutations in either enzyme result in a severe combined immunodeficiency (SCID). Confusingly, the same abbreviation (PNPase), is also used for another, otherwise unrelated, enzyme, namely Polynucleotide Phosphorylase.
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Product Overview
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Purine Nucleoside Phosphorylase produced in microorganism has a molecular mass of 32 kDa.
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Synonym
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inosine phosphorylase; PNPase; PUNPI; PUNPII; inosine-guanosine phosphorylase; nucleotide phosphatase; purine deoxynucleoside phosphorylase; purine deoxyribonucleoside phosphorylase; purine nucleoside phosphorylase; purine ribonucleoside phosphorylase; purine-nucleoside:phosphate ribosyltransferase.
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CAS Number
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9030-21-1
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Physical form
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White powder, lyophilized
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Isoelectric point
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6.0
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Optimum pH
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7.5~ 8.0
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Michaelis constant
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2.2×10-4M(Inosine)
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pH Stability
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5.0~10.0
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Optimum temperature
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60℃
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Reaction
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purine nucleoside + phosphate = purine + α-D-ribose 1-phosphate
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Thermal Stability
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< 55℃ (pH7.7, 30min)
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Inhibitors
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Ag+, Hg2+
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Specific Activity
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≥ 200U/mg protein
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Unit Definition
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One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH7.7 at 37℃.
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Preparation Instructions
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The enzyme is reconstituted in 50mM potassium phosphate buffer, pH 7.7 for activity assay.
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Storage Temperature
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-20℃
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Gene Information
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Gene Name
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NP(Homo sapiens)
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Synonyms
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nucleoside phosphorylase; PNP; PUNP; PRO1837; FLJ94043; FLJ97288; FLJ97312; MGC117396; MGC125915; MGC125916; PNPH_HUMAN; Purine nucleoside phosphorylase; EC 2.4.2.1; Inosine phosphorylase.
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GeneID
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4860
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mRNA Refseq
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NM_000270
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Protein Refseq
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NP_000261
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MIM
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164050
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UniProt ID
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P00491
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Chromosome Location
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14q13.1
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Pathway
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Nicotinate and nicotinamide metabolism;Purine metabolism;Pyrimidine metabolism;Nucleotide metabolism
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Function
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purine-nucleoside phosphorylase activity; purine-nucleoside phosphorylase activity; purine-nucleoside phosphorylase activity.
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Crystal structure of PNP
PDB rendering based on 1m73. (Homo sapiens)
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Download Datasheet:
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