|
Cat. No.
|
CED021
|
|
Description
|
The enzyme horseradish peroxidase (HRP), found in horseradish, is used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. HRP is often used in conjugates (molecules that have been joined genetically or chemically) to determine the presence of a molecular target. For example, an antibody conjugated to HRP may be used to detect a small amount of a specific protein in a western blot. Here, the antibody provides the specificity to locate the protein of interest and the HRP enzyme, in the presence of a substrate, produces a detectable signal. Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry.
|
|
Product Overview
|
Peroxidase produced in horseradish has a molecular mass of 40 kDa.
|
|
Synonym
|
myeloperoxidase; lactoperoxidase; verdoperoxidase; guaiacol peroxidase; thiocyanate peroxidase; eosinophil peroxidase; Japanese radish peroxidase; horseradish peroxidase (HRP); extensin peroxidase; heme peroxidase; MPO; oxyperoxidase; protoheme peroxidase; pyrocatechol peroxidase; scopoletin peroxidase; donor:hydrogen-peroxide oxidoreductase
|
|
CAS Number
|
9003-99-0
|
|
Physical form
|
Reddish-brown amorphous powder, lyophilized
|
|
Isoelectric point
|
7.2
|
|
Optimum pH
|
6.5
|
|
Stability
|
Stable at -20℃ for at least one year
|
|
pH Stability
|
5.0~10.0
|
|
Reaction
|
donor + H2O2 = oxidized donor + 2 H2O
|
|
Optimum temperature
|
45℃
|
|
Thermal Stability
|
< 50℃ (pH6.0,15min)
|
|
Inhibitors
|
Cyanide, sulfide, fluoride, azide
|
|
Specific Activity
|
≥250 Purpurogallin U/mg solid
|
|
Unit Definition
|
One purpurogallin unit causes the formation of one milligram of purpurogallin in 20s at pH 6.0 at 20 °C.
|
|
Preparation Instructions
|
The enzyme is reconstituted in 100mM phosphate buffer, pH 6.0 for activity assay.
|
|
Storage Temperature
|
-20℃
|
|
|
Download Datasheet:
|
|