Catalog # or name ...
Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5,10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2
L-Pipecolic Acid Oxidase; Sarcosine-Oxygen Oxidoreductase (Demethylating); Oxidoreductases, N-Demethylating
Yellowish powder, lyophilized
One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8.0 at 37℃.
65 kDa (Gel filtration)
<40℃ (pH7.0, 30min)
Ag+, Hg2+, Cu2+ are strongly inhibiting. The even stronger inhibiting effect of thiol group suggests that SDS might play a role in the active site of the enzyme.
The enzyme is reconstituted in 20mM Tris-HCl buffer, pH 8.0 for activity assay.
SAO (Bos taurus)
Amine oxidase activity; oxidoreductase activity; calcium ion binding; copper ion binding;quinone binding
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