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Cat. No.
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CED023
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Description
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Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5,10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2
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CAS No.
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9029-22-5
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Synonym
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L-Pipecolic Acid Oxidase; Sarcosine-Oxygen Oxidoreductase (Demethylating); Oxidoreductases, N-Demethylating
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Physical form
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Yellowish powder, lyophilized
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Specific Activity
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≥8U/mg protein
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Unit definition
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One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8.0 at 37℃.
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Molecular Weight
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65 kDa (Gel filtration)
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Isoelectric point
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5.0
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Michaelis constant
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2.7×10-3M(Sarcosine)
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Optimum pH
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8.0
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Optimum temperature
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40℃
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pH Stability
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7.0~9.0 (25°C,20hr)
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Storage Temperature
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-20°C
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Thermal Stability
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<40℃ (pH7.0, 30min)
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Inhibitors
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Ag+, Hg2+, Cu2+ are strongly inhibiting. The even stronger inhibiting effect of thiol group suggests that SDS might play a role in the active site of the enzyme.
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Preparation Instructions
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The enzyme is reconstituted in 20mM Tris-HCl buffer, pH 8.0 for activity assay.
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Gene Information
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Gene Name
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SAO (Bos taurus)
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Gene Alias
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BSAO
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GeneID
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789307
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mRNA Refseq
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NM_001130764
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Protein Refseq
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NP_001124236
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UniProt ID
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Q29437
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Chromosome Location
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19
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Function
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Amine oxidase activity; oxidoreductase activity; calcium ion binding; copper ion binding;quinone binding
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Download Datasheet:
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