The CA72-4 is a tumor associated antigen with a molecular weight of >1000Kd. LS-174Tcarcinoma cells.
Mouse. Hybridization of P3-X63-Ag8.653 .myeloma cells with spleen cells from Balb/c mice.
BALB/C mce were injected with membrane enriched fraction of a human LS-174T metastasis.
>90% pure (SDS-PAGE). Protein A chromatography.
1 x 10-10mol/l.
10mM Sodium Phosphate, 0.15M Sodium Chloride, pH 7.4
Immunohistochemistry on frozen or paraffin sections. Each laboratory should determine an optimum working titer for use in its particular application. Recommended antibody pair for immunoassay: Capture Detection CAB047 DCABH-20088
Upon receipt, aliquot and store at <–40°C. Avoid multiple freeze/thaw cycles. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used.