The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors and consists of only eight amino acids. This peptide can be expressed and detected with the protein of interested as an amino-terminal or carboxy-terminal fusion
A synthetic peptide (DYKDDDDK) coupled to KLH
Recognises over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins.
Mouse Monoclonal IgG1κ
Western blot: 1:1,000; Immunoprecipitation: 1:200; Immunostaining: 1:200
Presentation and storage:
Lyophilized powder. Reconstitute it with sterile water to 1 mg/ml. Stable for one year at -20℃from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Stored at 4℃, the aliquot will remain stable for three months