"Casp3" Related Products

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Recombinant Mouse Casp3 protein

Cat.No.: Casp3-1643M
Product Overview: Recombinant Mouse Casp3 protein was expressed in E. coli.
Source: E. coli
Species: Mouse
Bio-activity: 300000 units/mg
Molecular Mass: Of large (17 kD) and small (11 kD) subunits
Unit Definition: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Applications: Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
Notes: Centrifuge the vial prior to opening.
Storage: The lyophilized caspase-3 is stable for 1 year at –70 centigrade. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 centigrade. Avoid multiple freeze/thaw cycles as activity might decrease.
Reconstitution: Reconstitute to 1 unit per µl in PBS containing 15% glycerol
Gene Name: Casp3 caspase 3 [ Mus musculus ]
Official Symbol: Casp3
Synonyms: CASP3; caspase 3; caspase-3; SCA-1; CASP-3; CPP-32; protein Yama; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; CC3; AC-3; Lice; Yama; mldy; CPP32; Apopain; Caspase-3; A830040C14Rik;
Gene ID: 12367
mRNA Refseq: NM_009810
Protein Refseq: NP_033940
MIM:
UniProt ID:
Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem
Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; cysteine-type peptidase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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