Recombinant Mouse Casp3 protein was expressed in E. coli.
Of large (17 kD) and small (11 kD) subunits
One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
Centrifuge the vial prior to opening.
The lyophilized caspase-3 is stable for 1 year at –70 centigrade. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 centigrade. Avoid multiple freeze/thaw cycles as activity might decrease.
Reconstitute to 1 unit per µl in PBS containing 15% glycerol