Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Recombinant Mouse Casp3 protein

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GST(1)
His(8)
Met&His(1)
N/A(4)
Cat.No.:
Casp3-1643M
Product Overview:
Recombinant Mouse Casp3 protein was expressed in E. coli.
Source:
E. coli
Species:
Mouse
Bio-activity:
300000 units/mg
Molecular Mass:
Of large (17 kD) and small (11 kD) subunits
Unit Definition:
One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Applications:
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
Notes:
Centrifuge the vial prior to opening.
Storage:
The lyophilized caspase-3 is stable for 1 year at –70 centigrade. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 centigrade. Avoid multiple freeze/thaw cycles as activity might decrease.
Reconstitution:
Reconstitute to 1 unit per µl in PBS containing 15% glycerol


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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