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Cat. No.
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CHEK1-4838H
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Product Overview
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Recombinant
full-length human CHEK1 was expressed by baculovirus in Sf9 insect cells
using a N-terminal GST tag.
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Description
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CHEK1 is a 56 kd
serine/threonine protein kinase that was originally identified in fission
yeast to play a role in activation of the DNA damage checkpoint in the G2
phase of the cell cycle. CHEK1 appears to function downstream of several of
the known fission yeast checkpoint gene products, including that encoded by
rad3+, a gene with sequence similarity to the ATM gene mutated in patients
with ataxia telangiectasia.
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Applications
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Kinase Assay;
Western Blot
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Molecular Weight
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82 kDa
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Expression System
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Sf9 insect cells
using baculovirus
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Species
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Human
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Form
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Recombinant protein
stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 Mm
EDTA, 0.1 mM PMSF, 25 % glycerol.
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Specific Activity
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214 nmol/min/mg
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Purity
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>
95 %
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Concentration
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0.1 ug/ul
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Sequences
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Full-length
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Storage
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Store
product at -70oC. For optimal storage, aliquot target into
smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple
freeze/thaw cycles.performance, avoid repeated handling and multiple
freeze/thaw cycles.
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Antigen Gene Information
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Gene Name
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CHEK1 CHK1 checkpoint homolog (S. pombe) [ Homo
sapiens ]
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Official Symbol
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CHEK1
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Synonyms
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CHEK1;
CHK1 checkpoint homolog (S. pombe); CHK1; CHK1 (checkpoint, S. pombe)
homolog; Serine/threonine-protein kinase Chk1; EC 2.7.11.1;
serine/threonine-protein kinase Chk1; OTTHUMP00000231300; OTTHUMP00000231301;
OTTHUMP00000231306; OTTHUMP00000231307; OTTHUMP00000231309; Checkpoint, S.
pombe, homolog of, 1
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GeneID
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1111
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mRNA Refseq
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NM_001114121
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Protein Refseq
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NP_001107593
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MIM
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603078
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UniProt ID
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O14757
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Chromosome Location
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11q24.2
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Pathways
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Activation
of ATR in response to replication stress; Cell cycle; p53 signaling pathway;
Cell Cycle Checkpoints; IL-3 Signaling Pathway; HTLV-I infection; Ubiquitin
Mediated Degradation of Phosphorylated Cdc25A
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Function
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ATP
binding; nucleotide binding; protein binding; protein kinase activity; protein
serine/threonine kinase activity
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.jpg)
PDBrendering based on 1ia8.
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