Recombinant Human Euchromatic Histone-lysine N-methyltransferase 2, GST-tagged

• Cat.No.: EHMT2-1582H
• Product Overview: Recombinant human EHMT2 (807-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: EHMT2-1582H
• Description: EHMT2 is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
• Applications: Western Blot
• Molecular Weight: 73 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Sequences: 807-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Lysine degradation; RNA Polymerase I Promoter Clearance; RNA Polymerase I Transcription; RNA Polymerase I Transcription Initiation; Regulation of Androgen receptor activity; Transcription

Gene Information

• Gene Name: EHMT2 euchromatic histone-lysine N-methyltransferase 2 [ Homo sapiens ]
• Official Symbol: EHMT2
• Synonyms: EHMT2; euchromatic histone-lysine N-methyltransferase 2; G9A; BAT8; NG36; KMT1C; C6orf30; FLJ35547; DKFZp686H08213; histone-lysine N-methyltransferase EHMT2; H3-K9-HMTase 3; OTTHUMP00000029262; G9A histone methyltransferase; H3-K9-HMTase 3; HLA-B associated transcript 8; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; lysine N-methyltransferase 1C; protein G9a; EC 2.1.1.43
• Gene ID: 10919
• mRNA Refseq: NM_006709
• Protein Refseq: NP_006700
• MIM: 604599
• UniProt ID: Q96KQ7
• Chromosome Location: 6p21.31
• Function: histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding