Recombinant Human Mdm2 P53 Binding Protein Homolog (Mouse), GST-tagged
Cat.No. : | MDM2-4858H |
Product Overview : | Recombinant human MDM2 (202-end) was expressed in E. coli cells using an N-terminal GST tag. |
- Specification
- Gene Information
- Related Products
Cat. No. : | MDM2-4858H |
Description : | MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by p53, as part of an autoregulatory negative feedback loop. Overexpression of the MDM2 gene product can lead to excessive inactivation of p53 thereby diminishing its tumor suppressor function. The inactivation of p53 is mediated by the E3 ubiquitin ligase activity of MDM2 which targets p53 for proteasomal degradation. MDM2 also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Amplification of MDM2 is frequently observed in human sarcomas and this is consistent with the hypothesis that MDM2 binds to p53 which then leads to escape from p53-regulated growth control. |
Applications : | Kinase Assay; Western Blot |
Molecular Weight : | 84 kDa |
Expression System : | E. coli |
Species : | Human |
Form : | Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Purity : | > 75% |
Concentration : | 0.2 ug/ul |
Sequences : | 202-end |
Storage : | Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Pathways : | Bladder cancer; Cell cycle; Chronic myeloid leukemia; Endocytosis; Glioma; Melanoma; Pathways in cancer; Prostate cancer; Ubiquitin mediated; proteolysis; p53 signaling pathway |
Gene Name : | MDM2 Mdm2 p53 binding protein homolog (mouse) [ Homo sapiens ] |
Official Symbol : | MDM2 |
Synonyms : | MDM2; Mdm2 p53 binding protein homolog (mouse); HDMX; hdm2; MGC5370; MGC71221; mouse double minute 2 homolog; OTTHUMP00000183495; p53-binding protein MDM2; ubiquitin-protein ligase E3 Mdm2; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; HDM2; EC 6.3.2.-; Hdm2; Double minute 2 protein |
Gene ID : | 4193 |
mRNA Refseq : | NM_001145337 |
Protein Refseq : | NP_001138809 |
MIM : | 164785 |
UniProt ID : | Q00987 |
Chromosome Location : | 12q13-q14 |
Function : | basal transcription repressor activity; enzyme binding; identical protein binding; ligase activity; metal ion binding; p53 binding; ubiquitin-protein ligase activity; zinc ion binding |
Products Types
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Mdm2-1771M | Recombinant Mouse Mdm2 Protein, His-tagged | +Inquiry |
MDM2-2531R | Recombinant Rhesus Macaque MDM2 Protein, His (Fc)-Avi-tagged | +Inquiry |
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◆ Lysates | ||
MDM2-4404HCL | Recombinant Human MDM2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.
The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.
Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.
Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.
Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.
The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.
Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.
Customer Reviews (3)
Write a reviewValidated in multiple assays.
Great for cell culture.
Consistent performance, highly reproducible.
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