Recombinant Human Protein Arginine Methyltransferase 1, GST-tagged

• Cat.No.: PRMT1-1616H
• Product Overview: Recombinant full-length human PRMT1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: PRMT1-1616H
• Description: PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies.
• Applications: Western Blot
• Molecular Weight: 67 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol, 0.1 mM EDTA.
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Androgen Receptor Signaling Pathway; Direct p53 effectors; Energy Metabolism; Tryptophan metabolism; mRNA processing

Gene Information

• Gene Name: PRMT1 protein arginine methyltransferase 1 [ Homo sapiens ]
• Official Symbol: PRMT1
• Synonyms: PRMT1; protein arginine methyltransferase 1; ANM1; HCP1; IR1B4; HRMT1L2;protein arginine N-methyltransferase 1; OTTHUMP00000236172; OTTHUMP00000236176; OTTHUMP00000236178; OTTHUMP00000236179; HMT1 hnRNP methyltransferase-like 2; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; NP_938074.2; EC 2.1.1.125
• Gene ID: 3276
• mRNA Refseq: NM_198318
• Protein Refseq: NP_938074
• MIM: 602950
• UniProt ID: Q8WUW5
• Chromosome Location: 19q13.3
• Function: N-methyltransferase activity; core promoter proximal region DNA binding; enzyme binding; histone methyltransferase activity; histone methyltransferase activity (H4-R3 specific); methyltransferase activity; protein binding; transferase activity