Full-length recombinant human PTEN was expressed in E. coli cells using an N-terminal GST tag.
PTEN is a tumor suppressor that is frequently mutated in a large number of cancers. PTEN has phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity and contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases.
Phosphatase Assay; Western Blot
Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Endometrial cancer; Focal adhesion; Glioma; Inositol phosphate metabolism; Melanoma; Pathways in cancer; Phosphatidylinositol signaling; Prostate cancer; Small cell lung cancer; Tight junction; p53 signaling pathway; Signaling in Immune system; Signalling by NGF; PIP3 activates AKT signaling