Recombinant Human Mutant Tumor Necrosis Factor Alpha
| Cat.No. : | TNF-25H |
| Product Overview : | The clinical use of the potent anti-tumor activity of TNF-alpha has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-a, which binds to the murine TNF-R55 but not to the mouse TNF-R75, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-a mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo. Recombinant Human TNF-alpha Variant/Mutant compared with the wild-type, has an amino acid sequence deletion from a.a. 1-7, and the following a.a. substitutes Arg8, Lys9, Arg10 and Phe157 which is proven to have more activity and with less inflammatory side effect in vivo. Recombinant Human TNF-alpha Mutant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 151 amino acids and having a molecular mass of 16,886 Dalton. |
- Specification
- Gene Information
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| Cat. No. : | TNF-25H |
| Description : | TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF. |
| Purity : | >95% as determined by SEC-HPLC and SDS-PAGE . |
| Formulation : | Lyophilized from a 0.2 µm filtered solution in PBS. |
| Specific Activity : | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.01 ng/ml, corresponding to a Specific Activity of 1.0 x 108IU/mg. |
| Endotoxin : | Less than 0.01ng/µg (0.01IEU/µg) determined by LAL test. |
| Reconstitution : | It is recommended to reconstitute the lyophilized TNF-alpha Mutant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storagee : | Lyophilized samples are stable for up to twelve months from date of receipt at -20°C to -70°C. Aliquot to avoid repeated freeze-thaw cycles. |
| Gene Name : | TNF |
| Synonyms : | tumor necrosis factor (TNF superfamily, member 2); DIF; TNFA; TNFSF2; TNF-alpha; Cachectin; OTTHUMP00000037669; TNF-a; APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor precursor; tumor necrosis factor; tumor necrosis factor (cachectin); tumor necrosis factor alpha; tumor necrosis factor, alpha (cachectin); TNF |
| Gene ID : | 7124 |
| mRNA Refseq : | NM_000594.2 |
| Protein Refseq : | NP_000585.2 |
| MIM : | 191160 |
| UniProt ID : | P01375 |
| Chromosome Location : | 6p21.3 |
| Function : | Cytokine activity, Identical protein binding, Tumor necrosis factor receptor binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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