Recombinant Human Sirtuin 2, His-tagged

• Cat.No.: SIRT2-4875H
• Product Overview: Recombinant full length human SIRT2 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Cat. No.: SIRT2-4875H
• Description: SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington" s disease. Huntington"s disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition.
• Applications: SIRT Assay
• Molecular Weight: 42 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Specific Activity: 8000 RLU/min/mg
• Purity: > 70 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Signaling events mediated by HDAC Class I; Signaling events mediated by HDAC Class III

Gene Information

• Gene Name: SIRT2 sirtuin 2 [ Homo sapiens ]
• Official Symbol: SIRT2
• Synonyms: SIRT2; sirtuin 2; SIR2; SIR2L; SIR2L2; FLJ35621; FLJ37491; NAD-dependent deacetylase sirtuin-2; sirtuin type 2; OTTHUMP00000197460; OTTHUMP00000197461; OTTHUMP00000197465; SIR2-like protein 2; sir2-related protein type 2; silent information regulator 2; EC 3.5.1.-; sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 2
• Gene ID: 22933
• mRNA Refseq: NM_012237
• Protein Refseq: NP_036369
• MIM: 604480
• UniProt ID: Q8IXJ6
• Chromosome Location: 19q13
• Function: NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; histone acetyltransferase binding; histone deacetylase binding; hydrolase activity; metal ion binding; transcription factor binding; tubulin deacetylase activity; ubiquitin binding; zinc ion binding; protein binding; protein deacetylase activity