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Recombinant Human IGFBP5 protein

Cat.No. : IGFBP5-5052H
Product Overview : Recombinant Human IGFBP5 protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Description : An IGFBP5 gene was recently identified as being important for adaptation to varying water salinity in fish.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Molecular Mass : Approximately 28.6 kDa, a single non-glycosylated polypeptide chain containing 252 amino acids.
Protein length : 252
AA Sequence : LGSFVHCEPCDEKALSMCPPSPLGC ELVKEPGCGCCMTCALAEGQSCGVY TERCAQGLRCLPRQDEEKPLHALLH GRGVCLNEKSYREQVKIERDSREHE EPTTSEMAEETYSPKIFRPKHTRIS ELKAEAVKKDRRKKLTQSKFVGGAE NTAHPRIISAPEMRQESEQGPCRRH MEASLQELKASPRMVPRAVYLPNCD RKGFYKRKQCKPSRGRKRGICWCVD KYGMKLPGMEYVDGDFQCHTFDSSN VE
Endotoxin : Less than 0.1 EU/μg of rHuIGF-BP5 as determined by LAL method.
Purity : >96% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IGFBP5
Official Symbol : IGFBP5
Synonyms : IGFBP5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5; IBP5;
Gene ID : 3488
mRNA Refseq : NM_000599
Protein Refseq : NP_000590
MIM : 146734
UniProt ID : P24593

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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