Active Recombinant Human Neurotrophin 3

• Cat.No.: NTF3-044H
• Product Overview: Recombinant human NT-3 was expressed in E. coli and purified by SDS PAGE and HPLC, 13.6 KDa.

Specification

• Cat. No.: NTF3-044H
• Description: NT-3 is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, Mouse and rat NT-3 are identical.
• Source: Human
• Host: E.coli
• Form: Supplied lyophilized with no additives. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Allow to set for at least 30 minutes, mix well.
• Purity: > 98% pure by SDS PAGE and HPLC
• Sequence: Region of NT3 protein corresponding to amino acids MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT.
• Molecular Mass: 13.6 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.
• Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: NTF3 neurotrophin 3 [ Homo sapiens ]
• Official Symbol: NTF3
• Synonyms: neurotrophin 3; NT3; HDNF; NGF2; NGF-2; MGC129711; NTF3; NT-3; Nerve growth factor 2; Neurotrophic factor
• Gene ID: 4908
• mRNA Refseq: NM_001102654
• Protein Refseq: NP_001096124
• MIM: 162660
• UniProt ID: P20783
• Chromosome Location: 12p13
• Pathway: MAPK signaling pathway; Neurotrophic factor-mediated Trk receptor signaling; Neurotrophin signaling pathway; Trk receptor signaling mediated by the MAPK pathway; p75(NTR)-mediated signaling
• Function: growth factor activity; nerve growth factor binding; neurotrophin receptor binding; receptor binding