Active Recombinant Rat Uchl1 protein(Glu2-Ala223), His-tagged

• Cat.No.: Uchl1-1809R
• Product Overview: Recombinant Rat UCHL1 (Q00981) (Glu 2-Ala 223) was expressed in E. coli, fused with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Rat
• Tag: N-His
• Protein length: Glu2-Ala223
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The recombinant Rat UCHL1 comprises 233 amino acids and predicts a molecular mass of 26.2 kDa. The apparent molecular mass of the Rat UCHL1 is approximately 28 kDa in SDS-PAGE under reducing conditions.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Uchl1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) [ Rattus norvegicus ]
• Official Symbol: Uchl1
• Synonyms: UCHL1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; PGP9.5; UCH-L1; PGP 9.5; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin carboxy-terminal hydrolase L1
• Gene ID: 29545
• mRNA Refseq: NM_017237
• Protein Refseq: NP_058933