Active Recombinant Mouse Erbb2 protein, His-tagged

• Cat.No.: Erbb2-1152M
• Product Overview: Recombinant Mouse Erbb2 (Met 1-Thr 653) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to bind Anti-Erbb2 Antibody (Pertuzumab) in functional ELISA.
• Molecular Mass: The secreted recombinant mouse ERBB2 comprises 642 amino acids and has a calculated molecular mass of 71.1 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 100-110 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met 1-Thr 653
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Mus musculus ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); receptor tyrosine-protein kinase erbB-2; c-erbB-2; p185erbB2; Neu oncogene; proto-oncogene NEU; proto-oncogene c-ErbB-2; avian erythroblastosis oncogene B 2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; mKIAA3023
• Gene ID: 13866
• mRNA Refseq: NM_001003817
• Protein Refseq: NP_001003817