| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
C-hFc |
| Protein length : |
Met1-Val243 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×10E4 cells/well are added to mCD6-Fc coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately > 50% cells will adhere specifically after 60 minutes at 37°C. |
| Molecular Mass : |
The recombinant mouse CD6/Fc is a disulfide-linked homodimer. The reduced monomer comprises 621 amino acids and has a calculated molecular mass of 68.2 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 85 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
