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Cat. No.
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EIF2C3-5174H
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Product Overview
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A DNA sequence encoding
the human EIF2C3 ( Q9H9G7-1 ) ( Met 1 - Ala 860 ) was expressed, with a
polyhistidine tag at the N-terminus.
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Description
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Protein argonaute-3, also
known as EIF2C3, Eukaryotic translation initiation factor 2C 3, eIF-2C 3 AGO3
and EIF2C3, is cytoplasm protein which belongs to the argonaute family and
Ago subfamily. EIF2C3 / AGO3 contains one PAZ domain and one Piwi domain.
MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes
that post-transcriptionally regulate gene expression. It is becoming clear
that miRNAs play an important role in the regulation of gene translation
during development. EIF2C3 / AGO3 is required for RNA-mediated gene silencing
(RNAi). EIF2C3 / AGO3 binds to short RNAs such as microRNAs (miRNAs) and
represses the translation of mRNAs which are complementary to them. EIF2C3 /
AGO3 lacks endonuclease activity and does not appear to cleave target mRNAs.
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Source
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Human
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Host
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Baculovirus
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Form
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Lyophilized from
sterile 20mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Normally 5 % -
8 % trehalose and mannitol are added as protectants before lyophilization.
Specific concentrations are included in the hardcopy of COA.
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Purity
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> 88 % as
determined by SDS-PAGE
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Endotoxin
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< 1.0 EU per μg protein
as determined by the LAL method
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Stability
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Samples are stable
for up to twelve months from date of receipt at -70oC.
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Predicted
N terminal
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Met
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Molecular
Mass
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The recombinant human
EIF2C3 consists of 878 amino acids and predicts a molecular mass of 99.6 kDa.
It migrates as an approximately 90 kDa band in SDS-PAGE under reducing
conditions.
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Application
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SDS-PAGE
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Reconstitution
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A hardcopy of COA
with reconstitution instruction is sent along with the products.
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Storage
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Store it under
sterile conditions at -70oC upon receiving. Recommend to aliquot the protein
into smaller quantities for optimal storage. Avoid repeated freeze-thaw
cycles.
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Antigen Gene Information
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Gene
Name
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EIF2C3 eukaryotic translation initiation
factor 2C, 3 [ Homo sapiens ]
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Official
Symbol
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EIF2C3
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Synonyms
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EIF2C3;
eukaryotic translation initiation factor 2C, 3; AGO3; FLJ12765; MGC86946;
protein argonaute-3; hAgo3; eIF2C 3; eIF-2C 3; argonaute3; argonaute 3;
OTTHUMP00000008758; OTTHUMP00000008759; Argonaute3; Eukaryotic translation
initiation factor 2C 3
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GeneID
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192669
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mRNA
Refseq
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NM_024852
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Protein
Refseq
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NP_079128
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MIM
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607355
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UniProt
ID
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Q9H9G7
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Chromosome
Location
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1p34
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Pathways
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MicroRNA (miRNA)
Biogenesis; Regulatory RNA pathways
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Function
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RNA binding; protein
binding; translation initiation factor activity
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Download Datasheet:
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