Active Recombinant Rat Lcn2 protein(Met1-Asn198), His-tagged

• Cat.No.: Lcn2-5176R
• Product Overview: Recombinant Rat LCN2 (P30152) (Met 1-Asn 198) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Rat
• Tag: C-His
• Protein length: Met1-Asn198
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin2. It binds >1.0 μM of Fe(DHBA)3.
• Molecular Mass: The recombinant rat LCN2 comprises 188 amino acids with and has a predicted molecular mass of 21.9 kDa. It migrates as an approximately 25 kDa band in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lcn2 lipocalin 2 [ Rattus norvegicus ]
• Official Symbol: Lcn2
• Synonyms: LCN2; lipocalin 2; neutrophil gelatinase-associated lipocalin; p25; NGAL; lipocalin-2; lipocalin 2 (oncogene 24p3); alpha-2U globulin-related protein; alpha-2-microglobulin-related protein; Sip24
• Gene ID: 170496
• mRNA Refseq: NM_130741
• Protein Refseq: NP_570097