| Cat. No. : |
Il1a-03M |
| Description : |
IL-1α is distinct from the other agonist member of the IL-1 family, IL-1β. Although IL-1α triggers the same IL-1 receptor and although many of the biological effects of IL-1α are similar to those of IL-1α, in humans IL-1α is predominantly an intracellular molecule. In fact, there is evidence that IL-1α has both intracellular functions as a precursor molecule due to a nuclear localization sequence. IL-1α as an unprocessed precursor is biologically as active as the processed form. IL-1α is also found constitutively in epithelial cells, whereas constitutive expression of IL-1β is rare. In many ways, IL-1α appears to be closer to the fibroblast growth factor family than the secreted IL-1β form. Therapeutic stratiegies for blocking IL-1β predominate over those for blocking IL-1α. Many humans have circulating neutralizing antibodies to IL-1α but not IL-1β. |
| Purity : |
>98% as determined by RP-HPLC, FPLC and SDS-PAGE. |
| Formulation : |
Mouse IL-1α was lyophilized after extensive dialysis against PBS. |
| Specific Activity : |
The ED50 as determined by the dose-dependant stimulation of mouse D10S cells was found to be less than 0.01 ng/ml, corresponding to a Specific Activity of 1.0 x 108IU/mg. |
| Endotoxin : |
Less than 0.1ng/µg (1 IEU/µg) of IL-1α. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized rmIL-1α in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : |
Lyophilized rmIL-1α although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rmIL-1α should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles. |
