Recombinant Mouse Interleukin-22
Cat.No. : | Il22-28M |
Product Overview : | Recombinant Mouse IL-22 produced in E. coli is a single, non-glycosylated polypeptide chain containing 147 amino acids and having a molecular mass of 16.7 kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Il22-28M |
Description : | Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family. |
Purity : | >97% as determined by RP-HPLC and SDS-PAGE. |
Formulation : | rmIL-22 was lyophilized after extensive dialysis against PBS. |
Specific Activity : | Recombinant Mouse IL-22 is fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-10 secretion in Colo205 cells is less than 0.5ng/ml, corresponding to a Specific Activity of 2.0 x 106IU/mg. |
Endotoxin : | Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
Reconstitution : | It is recommended to reconstitute the lyophilized rmIL-22 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage : | Lyophilized rmIL-22 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rmIL-22 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles. |
Gene Name : | Il22 |
Synonyms : | IL-22, IL-TIF, ILTIF, TIFa, IL-10-related T-cell-derived-inducible factor, ILTIFA, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23, Interleukin-22, IL-TIF alpha, Interleukin-22a, IL-22a, Interleukin-22 Precursor |
Gene ID : | 50929 |
mRNA Refseq : | NM_016971.1 |
Protein Refseq : | NP_058667.1 |
MIM : | 605330 |
UniProt ID : | Q9JJY9 |
Chromosome Location : | 10 67.0 cM |
Pathway : | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |
Function : | cytokine activity; protein binding |
Products Types
◆ Recombinant Protein | ||
Il22-242M | Active Recombinant Mouse Il22 Protein (Leu34-Val179), C-His tagged, Animal-free, Carrier-free | +Inquiry |
IL22-4375C | Recombinant Cynomolgus Monkey IL22 Protein | +Inquiry |
IL22-2301H | Recombinant Human IL22 Protein, His-tagged | +Inquiry |
IL22-360I | Active Recombinant Human IL22 Protein (147 aa) | +Inquiry |
IL22-44H | Recombinant Human IL22 Protein, C-6His tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
IL22-5232HCL | Recombinant Human IL22 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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