Recombinant Tobacco Etch Virus Protease
Cat.No. : | TEVProtease-07T |
Product Overview : | Recombinant Tobacco Etch Virus Protease produced in E.coli I has a molecular mass of approximately 27 kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | TEV Protease-07T |
Description : | Recombinant TEV Protease(rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C. rTEV contains both GST and His tag, and can be easily removed from the digestion reaction by affinity chromatography. |
Unit Definition : | One unit of rTEV cleaves ≥85% of 3 μg control substrate in 1 h at 30°C. |
10xTEV buffer : | 0.50 M Tris-HCl (pH 8.0), 10 mM DTT, 5 mM EDTA. |
Recommended Conditions for Cleavage of a Fusion Protein : | A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation time and/or more rTEV. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Storage : | Store rTEV at -70°C for long term or at -20°C for < 6 months. |
Products Types
◆ Recombinant Protein | ||
TEV Protease-1364T | Recombinant Tobacco etch virus TEV Protease Protein (G2038-N2273), His/GST-tagged | +Inquiry |
TEV protease-330T | Active Recombinant TEV protease protein, His-tagged | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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