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Active Recombinant Human EGFR, His-tagged

Cat.No. : EGFR-30H
Product Overview : Recombinant Human EGFR extracellular domain (Met 1-Ser 645) (NP_005219), fused with a C-terminal polyhistidine tag, was produced in Human Cell.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4
Bio-activity : 1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His, the EC50 of Human EGFR His is 100-500 ng/mL.
2.Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 µM as determined in an SPR assay (Biacore T200).
3.Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His & AVI Tag with an affinity constant of 5.81nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested).
Molecular Mass : The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
Protein Length : Met 1-Ser 645
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Publication :
Artificial antibody created by conformational reconstruction of the complementary-determining region on gold nanoparticles (2018)
PEGylation of Goldbody: PEG-aided conformational engineering of peptides on gold nanoparticles (2022)
Regulation of EGFR Protein Stability by the HECT-type Ubiquitin Ligase SMURF21,2 (2011)
Gene Name : EGFR epidermal growth factor receptor [ Homo sapiens ]
Official Symbol : EGFR
Synonyms : EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61;
Gene ID : 1956
mRNA Refseq : NM_005228
Protein Refseq : NP_005219
MIM : 131550
UniProt ID : P00533

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What biological processes of human embryonic development and tissue regeneration is EGFR associated with? 10/07/2019

It is associated with biological processes in human embryonic development and tissue regeneration.

Why does EGFR-inhibiting drug therapy have adverse reactions? 09/25/2019

EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.

What is the role of EGFR in tumor treatment? 08/27/2019

The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.

What are the general methods used for EGFR gene mutation testing? 03/22/2019

EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.

What other drugs can EGFR inhibitors be combined with to increase efficacy? 02/21/2019

The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.

How do EGFR mutations affect the efficacy of targeted therapies? 02/04/2019

EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.

Customer Reviews (3)

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Reviews
09/05/2022

    The powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.

    09/26/2019

      Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.

      03/23/2019

        The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.

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