Recombinant Human TNFRSF9, Fc-tagged expressed in human cells is a disulfide-linked homodimeric protein. The reduced monomer consists of 409 amino acids and has a calculated molecular mass of 45.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh4-1BB/Fc monomer is approximately 60-65 kDa due to glycosylation
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity.A DNA sequence encoding the N-terminal fragment (Met 1– Gln 186) of the extracellular domain of human 4-1BB (NP_001552.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
> 97%, as determined by SDS-PAGE and SEC-HPLC Analysis
Predicted N terminal:
< 1.0 EU per 1 μg of the cytokine as determined by the LAL method
Supplied as a 0.2μm filtered solution of PBS, pH7.4
Follow the instructions on the vial. Centrifuge the vial at 4°Cbefore opening to recover the entire contents.
Samples are stable for up to twelve months from date of receipt -70C
Store at –70°C; Avoid repeated freeze-thaw cycles