Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human TNFRSF9, Fc-tagged

Recombinant Human TNFRSF9, Fc-tagged

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Fc(10)
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Cat. No.:
TNFRSF9-43H
Product Overview:
Recombinant Human TNFRSF9, Fc-tagged expressed in human cells is a disulfide-linked homodimeric protein. The reduced monomer consists of 409 amino acids and has a calculated molecular mass of 45.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh4-1BB/Fc monomer is approximately 60-65 kDa due to glycosylation
Description:
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity.A DNA sequence encoding the N-terminal fragment (Met 1– Gln 186) of the extracellular domain of human 4-1BB (NP_001552.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 97%, as determined by SDS-PAGE and SEC-HPLC Analysis
Predicted N terminal:
Gln 25
Endotoxin:
< 1.0 EU per 1 μg of the cytokine as determined by the LAL method
Formulation:
Supplied as a 0.2μm filtered solution of PBS, pH7.4
Reconstitution:
Follow the instructions on the vial. Centrifuge the vial at 4°Cbefore opening to recover the entire contents.
Stability:
Samples are stable for up to twelve months from date of receipt -70C
Storage:
Store at –70°C; Avoid repeated freeze-thaw cycles


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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