Recombinant Human AIMP1 protein
Cat.No. : | AIMP1-87H |
Product Overview : | Recombinant Human AIMP1 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
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Description : | Endothelial-Monocyte Activating Polypeptide II (EMAP-II) is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 10⁴ IU/mg. |
Molecular Mass : | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
Protein length : | 166 |
AA Sequence : | SKPIDVSRLDLRIGCIITARKHPDA DSLYVEEVDVGEIAPRTVVSGLVNH VPLEQMQNRMVILLCNLKPAKMRGV LSQAMVMCASSPEKIEILAPPNGSV PGDRITFDAFPGEPDKELNPKKKIW EQIQPDLHTNDECVATYKGVPFEVK GKGVCRAQTMSNSGIK |
Endotoxin : | Less than 1 EU/μg of rHuEMAP-II as determined by LAL method. |
Purity : | >98% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | AIMP1 |
Official Symbol : | AIMP1 |
Synonyms : | AIMP1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; SCYE1, small inducible cytokine subfamily E, member 1 (endothelial monocyte activating); aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS interacting multifunctional protein 1; EMAP II; EMAP 2; EMAPII; p43; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; multisynthase complex auxiliary component p43; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); HLD3; EMAP2; SCYE1; |
Gene ID : | 9255 |
mRNA Refseq : | NM_001142415 |
Protein Refseq : | NP_001135887 |
MIM : | 603605 |
UniProt ID : | Q12904 |
Products Types
◆ Recombinant Protein | ||
AIMP1-130A | Active Recombinant Human AIMP1 Protein (166 aa) | +Inquiry |
AIMP1-865G | Recombinant Human Aminoacyl tRNA Synthetase Complex-interacting Multifunctional Protein 1, His-tagged | +Inquiry |
AIMP1-940H | Recombinant Human AIMP1, His-tagged | +Inquiry |
AIMP1-4429Z | Recombinant Zebrafish AIMP1 | +Inquiry |
SCYE1-2547H | Recombinant Human SCYE1, GST-tagged | +Inquiry |
◆ Lysates | ||
AIMP1-578HCL | Recombinant Human AIMP1 lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (11)
Ask a questionAIMP1 has been shown to affect cellular metabolism by regulating the activity of several metabolic enzymes. One of the known mechanisms of AIMP1-mediated metabolic regulation involves the inhibition of AMP-activated protein kinase (AMPK), a key regulator of cellular metabolism that responds to changes in energy balance. AIMP1 binds to and inhibits AMPK, leading to altered glucose and lipid metabolism in cells.
AIMP1 has potential as a diagnostic or prognostic marker for certain diseases, particularly cancer. Its expression has been shown to be increased in several types of cancer, and high levels of AIMP1 expression have been associated with poor prognosis in some cancers. Additionally, AIMP1 expression has been suggested as a biomarker for early detection and progression monitoring of certain cancers.
AIMP1 has been proposed as a potential therapeutic target for various diseases, including cancer and neurological disorders. However, more research is needed to fully understand the therapeutic potential of targeting AIMP1.
Mutations in the AIMP1 gene have been associated with various diseases, including rare neurological disorders such as leukodystrophies and movement disorders. Mutations in this gene have also been linked to susceptibility to certain types of cancers such as colorectal cancer and gastric cancer.
Mutations in AIMP1 have been linked to several neurological disorders, including Charcot-Marie-Tooth disease type 2D, hereditary sensory and autonomic neuropathy type 2, and peripheral neuropathy with or without agenesis of the corpus callosum. AIMP1 is thought to play a role in the maintenance of peripheral nerves, and mutations in this gene can lead to peripheral nerve degeneration.
AIMP1 protein expression and function are regulated by various mechanisms, including transcriptional regulation, post-transcriptional regulation, and post-translational modifications. For example, AIMP1 expression can be regulated by various transcription factors, such as PPARγ, NF-κB, and YY1. In addition, AIMP1 protein stability and activity can be regulated by phosphorylation, acetylation, and ubiquitination.
AIMP1 has been suggested as a potential therapeutic target in autoimmune diseases such as rheumatoid arthritis. Inhibition of AIMP1 has been shown to reduce the severity of arthritis in mouse models, possibly by reducing inflammation and modulating immune cell differentiation. However, more research is needed to determine the efficacy and safety of targeting AIMP1 for therapeutic purposes in humans.
The regulation of AIMP1 expression is complex and not yet fully understood. Multiple transcription factors, including NF-κB, p53, and c-Myc, have been shown to regulate AIMP1 expression by binding to its promoter region. In addition, epigenetic modifications, such as DNA methylation and histone acetylation, can also affect AIMP1 expression. Other factors, such as microRNA, can also regulate AIMP1 expression at the post-transcriptional level.
There are currently no drugs specifically targeting AIMP1 protein in development. But given the growing interest in AIMP1 as a therapeutic target, it is possible that such drugs may be developed in the future.
Various techniques can be used to study AIMP1 protein, including Western blot, immunofluorescence, co-immunoprecipitation, and mass spectrometry. These techniques can be used to study AIMP1 protein expression, localization, and protein-protein interactions.
Currently, there are no drugs that specifically target AIMP1. However, AIMP1 has been suggested as a potential therapeutic target for certain diseases, including cancer, and there is ongoing research to develop drugs that can modulate the activity of AIMP1. For example, small molecule inhibitors of AIMP1 have been identified and shown to have potential as cancer therapeutics in preclinical studies.
Customer Reviews (4)
Write a reviewheir dedicated team of experts is readily available to address any challenges or questions that may arise during my experiments.
Their profound knowledge and guidance provide invaluable assistance in overcoming hurdles and optimizing my research protocols, ensuring a seamless and efficient workflow.
Its exceptional purity, stability, and functionality make it a reliable tool for conducting precise and reliable research, opening up new avenues for exploration and discovery.
What truly sets this protein apart is the manufacturer's unwavering commitment to delivering exceptional technical support.
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