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Recombinant Ovine Prolactin Receptor

Cat.No. : PRLR-55O
Product Overview : Recombinant Ovine Prolactin Receptor Extra Celleular Domain produced inE.Coliis a non-glycosylated, Polypeptide chain containing 213 amino acids and having a molecular mass of 24.4 kDa. The Prolactin Receptor is purified by proprietary chromatographic techniques.
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Cat. No. : PRLR-55O
Description : Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.
Solubility : It is recommended to reconstitute the lyophilized PRL-R in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Ser-Pro-Pro-Glu.
Protein Content : Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280 nm using the absorbency value of 2.78 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PRL-R as a Reference Standard.
Storage : Lyophilized PRL-R although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prolactin Receptor should be stored at 4°Cbetween 2-7 days and for future use below -18°CFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : PRLR prolactin receptor [ Ovis aries ]
Synonyms : PRLR; prolactin receptor
Gene ID : 554331
Gene Name : PRLR prolactin receptor [ Ovis aries ]
Synonyms : PRLR; prolactin receptor
Gene ID : 554331

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does PRLR contribute to the development of breast cancer at the molecular level? 04/17/2022

PRLR overexpression or dysregulation has been associated with breast cancer. It can promote cell proliferation and survival through aberrant signaling pathways.

How does PRLR signaling vary in different tissues? 05/09/2021

Tissue-specific factors and the presence of co-receptors contribute to the diversity of PRLR signaling outcomes in different tissues and cell types.

What are the key signaling pathways activated by the PRLR protein? 02/22/2020

The JAK-STAT signaling pathway is a primary pathway activated by PRLR. It involves the activation of Janus kinases (JAKs) and signal transducers and activators of transcription (STATs).

Explain the process of endocytosis and intracellular trafficking of PRLR. 01/01/2020

PRLR undergoes endocytosis upon ligand binding, and the internalized receptor-ligand complex is trafficked through intracellular compartments, influencing downstream signaling and receptor recycling.

Discuss the post-translational modifications involved in regulating PRLR activity. 11/28/2018

Phosphorylation is a crucial post-translational modification of PRLR, affecting its activation and downstream signaling. Other modifications, such as glycosylation, also play roles.

Customer Reviews (3)

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Reviews
04/03/2022

    Consistent quality shines through—this protein has become indispensable.

    02/24/2021

      This protein streamlines our experiments.

      12/22/2018

        Fast shipping made this purchase convenient for our lab operations.

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