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Recombinant Human GAPDH, His-tagged

Cat.No. : GAPDH-9157H
Product Overview : Recombinant human GAPDH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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  • Gene Information
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Description : Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 1mM EDTA, 20% glycerol
Molecular Mass : 36 kDa (335aa), confirmed by MALDI-TOF.
Endotoxin : Less than 0.1 ng/μg (IEU/μg) of recombinant human GAPDH by the LAL method
Purity : More than 90%, as determined by SDS-PAGE
Stability : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
Official Symbol : GAPDH
Synonyms : GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; G3PD; MGC88685;
Gene ID : 2597
mRNA Refseq : NM_001256799
Protein Refseq : NP_001243728
MIM : 138400
UniProt ID : P04406
Chromosome Location : 12p13.31
Pathway : Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>
Function : NAD binding; NADP binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; oxidoreductase activity; peptidyl-cysteine S-nitrosylase activity; protein binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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