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Active Recombinant Rat Efnb3 protein, His-tagged

Cat.No. : Efnb3-8717R
Product Overview : Recombinant Rat Efnb3 protein(G3V7D4)(Met1-Ser224), fused with His tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Rat
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized rat EFNB3-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His, The EC50 of biotinylated mouse EphB3-His is 17.4-40.6 ng/ml.
Molecular Mass : The recombinant rat EFNB3 comprises 208 amino acids and predicts a molecular mass of 23.1 kDa. The apparent molecular mass of the recombinant protein is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Length : Met1-Ser224
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 85 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Efnb3 ephrin B3 [ Rattus norvegicus ]
Official Symbol : Efnb3
Synonyms : EFNB3; ephrin B3; ephrin ligand B3; ELK-L3; RGD1565678;
Gene ID : 360546
mRNA Refseq : NM_001100980
Protein Refseq : NP_001094450

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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