Active Recombinant Human IL3, His-tagged
Cat.No. : | IL3-8862H |
Product Overview : | Recombinant Human IL3 was produced in the endosperm tissue of barley grain (Hordeum vulgare). |
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Description : | IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. |
Source : | Hordeum vulgare |
Species : | Human |
Predicted N Terminal : | His |
Form : | Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2 |
Bio-activity : | The bioactivity of recombinant human IL-3 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically< 0.2 ng/ml, corresponding to a specific activity of>5 x 10e6 units/mg. |
Molecular Mass : | Recombinant human IL-3 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.2 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 20-23 kDa in SDS-PAGE |
Endotoxin : | Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay. |
Purity : | >95% by SDS-PAGE gel analysis. |
Storage : | The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ] |
Official Symbol : | IL3 |
Synonyms : | IL3; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; hematopoietic growth factor; IL 3; mast cell growth factor; MCGF; MGC79398; MGC79399; MULTI CSF; multilineage colony stimulating factor; P cell stimulating factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; IL-3; MULTI-CSF; |
Gene ID : | 3562 |
mRNA Refseq : | NM_000588 |
Protein Refseq : | NP_000579 |
MIM : | 147740 |
UniProt ID : | P08700 |
Chromosome Location : | 5q23-q31 |
Pathway : | Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; |
Function : | cytokine activity; growth factor activity; interleukin-3 receptor binding; |
Products Types
◆ Recombinant Protein | ||
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Il3-188M | Recombinant Active Mouse IL3 Protein, His-tagged(C-ter) | +Inquiry |
Il3-1037R | Recombinant Rat Il3 Protein, His-tagged | +Inquiry |
Il3-247I | Active Recombinant Rat Il3 Protein, His-tagged | +Inquiry |
Il3-107M | Active Recombinant Mouse Il3 Protein | +Inquiry |
◆ Lysates | ||
IL3-607HCL | Recombinant Human IL3 cell lysate | +Inquiry |
IL3-445RCL | Recombinant Rat IL3 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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