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Cat.No.
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YFP-10
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Product Name
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Recombinant Yellow Fluorescent Protein?
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Product Overview
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The recombinant YFP is expressed and purified from transformed?E. coli?using a method that ensures high purity and maximal YFP fluorescence.?
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Description
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Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a monomer with an N-terminus His-Tag with Ex./Em. = 525/538 nm, extinction coefficient 27390 (at 280 nm).
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Source
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E. coli
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Form
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Lyophilized
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Molecular Mass
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26.4 kDa
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AA Sequence
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MRGSGSSGAL LFHGKIPYVV EMEGNVDGHT FSIRGKGYGD ASVGKVDAQF ICTTGDVPVP WSTLVTTLTY GAQCFAKYGP ELKDFYKSCM
PDGYVQERTI TFEGDGNFKT RAEVTFENGS VYNRVKLNGQ GFKKDGHVLG KNLEFNFTPH CLYIWGDQAN HGLKSAFKIC HEITGSKGDF
IVADHTQMNT PIGGGPVHVP EYHHMSYHVK LSKDVTDHRD NMSLKETVRA VDCRKTYL?
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Endotoxin
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<0.1 ng/μg of YFP
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Purity
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Greater than 97% by SDS-PAGE and HPLC
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Applications
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The protein is suitable as control reagent for YFP expression studies or as labeling
reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of
YFP transfected cells, label other proteins, calibration of fluorometers and flow
cytometers, fluorescence microscope, or microinjection of YFP into cells and tissues, etc.
The recombinant YFP is ideal for fusion tag applications and is perfect for triple labeling
with EGFP and RFP.
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Storage
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Store at -80°C. Avoid repeated freeze-thaw cycles.
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Reconstitution
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Add sterile distilled water to the vial to fully solubilize the protein to a concentration of 1.0 mg/mL
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Download Datasheet:
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siz^xp; x???ily: Arial">Product Name
Recombinant GFP Protein
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Product Overview
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Recombinant full length Aequorea victoria GFP; amino acids 1-238 (238 amino acids), 26.8kDa. Swiss ID = P42212.
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Description
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Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
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Source
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E. coli
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Form
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Liquid
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Molecular Mass
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26.8 KDa
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Protein length
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1-238
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AA Sequence
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MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
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Endotoxin
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Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
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Purity
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> 95 % by SDS-PAGE
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Applications
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SDS-PAGE; WB
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Storage
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Concentration
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1 mg/ml
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Storage Buffer
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Related Products - YFP
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