A DNA sequence encoding the MouseANGPTL4 (Gly24 - Ser410) with 6 His tag on the N-Terminus was expressed in E. coli. This protein was purified by Ni-NTA column.
Angiopoietin-related protein 4 is a protein that in humans is encoded by the ANGPTL4 gene. Alternatively spliced transcript variants encoding different isoforms have been described. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.
Lyophilized 100μg mouse ANGPTL4 in 100μl of TBS ( 20 mM Tris, 50mM NaCl, pH 8.0 ), Carry free.
>90% in SDS-PAGE gel
Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg /mL . Allow to set at least 30 minutes at 4 °C, mix well.
Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
QGRPAQP EPPRFASWDE MNLLAHGLLQ LGHGLREHVE RTRGQLGALE RRMAACGNAC QGPKGKDAPF KDSEDRVPEG QTPETLQSLQ TQLKAQNSKI QQLFQKVAQQ QRYLSKQNLR IQNLQSQIDL LAPTHLDNGV DKTSRGKRLP KMTQLIGLTP NATHLHRPPR DCQELFQEGE RHSGLFQIQP LGSPPFLVNC EMTSDGGWTV IQRRLNGSVD FNQSWEAYKD GFGDPQGEFW LGLEKMHSIT GNRGSQLAVQ LQDWDGNAKL LQFPIHLGGE DTAYSLQLTE PTANELGATN VSPNGLSLPF STWDQDHDLR GDLNCAKSLS GGWWFGTCSH SNLNGQYFHS IPRQRQERKK GIFWKTWKGR YYPLQATTLL IQPMEATAAS