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Cat. No.
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IL10-40H
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Product Overview
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Recombinant Human Interleukin-10 produced in CHO is a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18606.29 Dalton. The IL-10 is purified by proprietary chromatographic techniques.
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Description
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IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
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Source
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Chinese Hamster Ovary Cells
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Amino Acid Sequence
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The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
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Formulation
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The protein was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5, 50mg BSA and 100mM NaCl.
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Solubility
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It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Biological Activity
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The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 107 IU/mg.
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Storage
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Lyophilized Interleukin 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL10 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.
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Gene Information
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Gene Name
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IL10 interleukin 10 [ Homo sapiens ]
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Synonyms
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CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; IL10; interleukin 10; cytokine synthesis inhibitory factor; Interleukin-10; Cytokine synthesis inhibitory factor
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GeneID
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3586
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mRNA Refseq
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NM_000572
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Protein Refseq
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NP_000563
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MIM
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124092
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UniProt ID
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P22301
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Chromosome Location
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1q31-q32
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Pathway
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Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
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Function
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cytokine activity; protein binding ; interleukin-10 receptor binding; growth factor activity
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