Catalog # or name ...
Recombinant Human Interleukin-10 produced in CHO is a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18606.29 Dalton. The IL-10 is purified by proprietary chromatographic techniques.
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Chinese Hamster Ovary Cells
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
The protein was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5, 50mg BSA and 100mM NaCl.
It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 107 IU/mg.
Lyophilized Interleukin 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL10 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.
IL10 interleukin 10 [ Homo sapiens ]
CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; IL10; interleukin 10; cytokine synthesis inhibitory factor; Interleukin-10; Cytokine synthesis inhibitory factor
Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
cytokine activity; protein binding ; interleukin-10 receptor binding; growth factor activity
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