Recombinant Human PTPRJ, GST-tagged

• Cat.No.: PTPRJ-206H
• Product Overview: Recombinant Human PTPRJ (a.a. 1024-1306) fused with GST-tag at N-terminal, was expressed in an E. coli expression system.

Specification

• Cat. No.: PTPRJ-206H
• Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells.
• Source: E. coli
• Molecular Mass: 58.9 kDa
• Purity: >95% by SDS-PAGE.
• SpecificActivity: 268 nmole/min/µg Assay conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 50 mM pNPP and DEP1. Assay was done at 30°C for 10 min, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ƐpNP- = 18,000 M-1cm-1
• Applications: Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
• Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Stability: >12 months at –80°C
• OfficialSymbol: PTPRJ

Gene Information

• Gene Name: PTPRJ protein tyrosine phosphatase, receptor type, J [ Homo sapiens ]
• Synonyms: protein tyrosine phosphatase, receptor type, J; DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA; PTPRJ; DEP-1; EC 3.1.3.48; Density-enhanced phosphatase 1;R-PTP-eta; R-PTP-J; HPTP eta;CD148 antigen density-enhanced phosphatase 1; protein-tyrosine phosphatase eta; human density enhanced phosphatase-1 protein-tyrosine phosphatase receptor type J; receptor-type tyrosine-protein phosphatase eta susceptibility to colon cancer 1, mouse, homolog of; protein tyrosine phosphatase, receptor type, J polypeptide
• Gene ID: 5795
• mRNA Refseq: NM_001098503
• Protein Refseq: NP_001091973
• MIM: 600925
• UniProt ID: Q12913
• Chromosome Location: 11p11.2
• Pathway: Adherens junction; PDGFR-beta signaling pathway; Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
• Function: beta-catenin binding; protein binding; delta-catenin binding; gamma-catenin binding