Trimeric form of Recombinant Human Adiponectin trimeric form was expressed in HEK293 cells. The cystenine 39 was replaced with Alanine (C39A) 9. hAd-C39A can only form trimer, but not hexamer or HMW form.
Adiponectin is a hormone exclusively expressed from adipose tissue. Many studies demonstrate that Adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle. Attenuate hepatic lipogenesis and gluconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells. In the circulation, Adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weight (MMW, also called trimer) forms 8. Different oligomeric complex of Adiponectin activates different signaling pathways and exerts distinct functions.
HEK293 cells (Human embryonic kidney cell line).
Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:
ETTTQGPGVL LPLPKGAATG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK.
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.05M NaCl, pH 7.2.
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Store lyophilized Adiponectin at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized AdipoQ remains stable until the expiry date when stored at -20°C.
ED50= 3-8.5 μg/mL, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF.
In vitro, ex vivo and in vivo activity analysis, binding assay et al.