Active Recombinant Human KIT, Fc Chimera

• Cat.No.: KIT-12H
• Product Overview: A DNA sequence encoding the signal peptide and extracellular domain of human Stem Cell Factor Receptor (amino acids 1-520) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.

Specification

• Description: Stem Cell Factor Receptor (SCFR) is a member of the receptor tyrosine kinase family. Binding of SCFR to its ligand Stem Cell Factor (PE-2008-5) induces receptor dimerization, autophosphorylation and signal transduction. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation and differentiation of mast cells, melanocytes and germ cells.
• Source: HEK293 Cells
• Species: Human
• Tag: Fc Chimera
• Form: The SCFR-Fc Chimera consists of 5-45% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Bio-activity: The ED50 of the SCFR-Fc Chimera is typically 1-3 ug/ml as measured by its ability to neutralize SCF mediated proliferation using the human growth factor dependent M-07e cell line.
• Molecular Mass: The SCFR-Fc Chimera migrates as a broad band between 90 and 150 kDa in SDS-PAGE due to post-translational modifications. This compares with the unmodified SCFR-Fc Chimera that has a predicted molecular mass of 83.1 kDa. The Chimera separates into a number of isoforms with a pI between 4.5 and 8 in 2D PAGE due to post-translational modifications. The unmodified SCFR–Fc Chimera has a predicted pI of 6.6.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KIT
• Synonyms: KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 164920
• UniProt ID: P10721
• Chromosome Location: 4q11-q12
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem
• Function: ATP binding; cytokine binding; metal ion binding; nucleotide binding; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity