Active Recombinant Human LCK, His-tagged

• Cat.No.: LCK-16H
• Product Overview: The recombinant Human LCK, His-tagged protein was expressed in E. coli and purified by affinity chromatography in combination with FPLC columns.

Specification

• Description: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.
• Source: E. coli
• Species: Human
• Tag: His
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Bio-activity: 1 unit equals 1 nanogram of purified protein.
• Molecular Mass: 59.8 kDa.
• AA Sequence: MGCGCSSHPE DDWMENIDVC ENCHYPIVPL DGKGTLLIRN GSEVRDPLVT YEGSNPPASP LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA QSLTTGQEGF IPFNFVAKAN SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS FLIRESESTA GSFSLSVRDF DQNQGEVVKH YKIRNLDNGG FYISPRITFP GLHELVRHYT NASDGLCTRL SRPCQTQKPQ KPWWEDEWEV PRETLKLVER LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHQRL VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLTINK LLDMAAQIAE GMAFIEERNY IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA KFPIKWTAPE AINYGTFTIK SDVWSFGILL TEIVTHGRIP YPGMTNPEVI QNLERGYRMV RPDNCPEELY QLMRLCWKER PEDRPTFDYL RSVLEDFFTA TEGQYQPQP
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: 10ng of LCK is sufficient to phosphorylated 1 ug of heat-inactivated nuclear or cytoplasmic extract at 30 C for 30 mins.
• Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: LCK lymphocyte-specific protein tyrosine kinase [ Homo sapiens ]
• Official Symbol: LCK
• Synonyms: LCK; lymphocyte-specific protein tyrosine kinase; tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; LSK; YT16; p56lck; pp58lck
• Gene ID: 3932
• mRNA Refseq: NM_005356
• Protein Refseq: NP_005347
• MIM: 608131
• UniProt ID: Q9H093
• Chromosome Location: 1q32.1
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity