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Recombinant Human CAD, His-tagged

Cat.No. : CAD-10649H
Product Overview : Recombinant Human CAD protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.
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Description : The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
Source : E.coli
Species : Human
Tag : His
Protein length : C-term-350a.a.
Storage : The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
Storage Buffer : 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
Gene Name : CAD carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase [ Homo sapiens ]
Official Symbol : CAD
Synonyms : CAD; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; CAD protein; CAD trifunctional protein; multifunctional protein CAD;
Gene ID : 790
mRNA Refseq : NM_004341
Protein Refseq : NP_004332
MIM : 114010
UniProt ID : P27708
Chromosome Location : 2p22-p21
Pathway : Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Pyrimidine biosynthesis, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem;
Function : ATP binding; aspartate binding; aspartate carbamoyltransferase activity; aspartate carbamoyltransferase activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; dihydroorotase

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What are the allosteric regulators of CAD, and how do they affect its activity? 08/07/2021

CAD is allosterically regulated by UTP, which inhibits its activity, and ATP, which activates it. These nucleotides bind to specific sites on CAD and modulate its function, helping to control pyrimidine biosynthesis.

What is the molecular function of CAD, and how does it play a central role in de novo pyrimidine biosynthesis? 03/22/2021

CAD is a multi-domain enzyme that catalyzes three sequential reactions in de novo pyrimidine biosynthesis: carbamoyl phosphate synthesis, aspartate carbamoylation, and dihydroorotate formation.

How does CAD contribute to maintaining nucleotide balance in the cell? 05/11/2020

CAD helps maintain nucleotide balance by ensuring an adequate supply of pyrimidine nucleotides. When the cell needs more pyrimidines, CAD activity increases, and when there is an excess, CAD is allosterically inhibited.

What are some general techniques used to study CAD at the molecular level? 06/01/2019

Researchers use techniques like X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and enzyme assays to study CAD's structure and function. These methods provide insights into its bio-molecular chemistry.

What is the allosteric regulation of CAD by UTP and ATP, and how do these nucleotides affect its activity? 10/04/2018

CAD is allosterically regulated by UTP and ATP. UTP negatively regulates CAD, while ATP positively regulates it. UTP binds to an allosteric site, promoting the formation of the less active CAD tetramer, whereas ATP stabilizes the more active CAD dimer.

Customer Reviews (3)

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Reviews
04/03/2021

    Product arrived quickly and as described.

    02/09/2020

      This product works wonders.

      09/23/2019

        Quick shipping and well-packaged.

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